Get Access Chromatography Of Photosynthetic Pigments In Spinach Biology Essay The purpose of this experiment was to separate and insulate the different photosynthetic pigments, found on Spinacia oleracea foliages and to pull out them utilizing the paper chromatography method. The purpose was besides to find the comparative sums of chlorophyll a and chlorophyll B from their soaking up spectra.
Rate of photosynthesis was determined at pH 7, 0. The amount of oxygen production was determined after five minutes at each light intensity.
Spinach leaf segments were infiltrated with 0. The rate of oxygen production increased relatively rapidly at lower light intensities but leveled off at intensities greater than lux, suggesting that light intensity is a limiting factor of photosynthesis below lux. At light intensities greater thenhowever, light intensity is no longer limiting.
Instead other factors such as amount of water, NaHCO3, chlorophyll, and other reagents of photosynthesis become limiting. Photosynthesis is a process that underlies all life on Earth.
It is essential in creating the sugars that sustain life. By converting light energy into chemical energy in the form of carbohydrates, it not only sustains the The spinach leaf essay of photosynthetic organisms themselves called The spinach leaf essay, but full essay samples are available only for registered users autotrophsbut also provides food for the heterotrophs that consume autotrophs.
Overall, photosynthesis consists of two sets of reactions: In the light dependent reactions use energy from light is absorbed by chlorophyll. This powers the release of electrons from water, which are then transferred to the electron carrier, NADPH. Oxygen is a product of the light dependent reactions.
In contrast, the light-independent reactions result in the production of sugar from carbon dioxide. It is useful to study factors that affect rate of photosynthesis in order to determine the conditions of optimal photosynthetic activity and explain various characteristics of plants.
For example, understanding the affect of light intensity on rate of photosynthesis may reveal why shrubs in the shade do not grow as tall as trees with greater exposure to sunlight. In this experiment, spinach leaves were subjected to varying light intensities to determine the effect of light intensity on rate of photosynthesis as measured by production of oxygen.
We expected rate of photosynthesis to increase with increasing light intensity. This was because increasing light intensity would increase the amount of energy available for the light dependent photosynthetic reactions. However, the increasing rate of photosynthesis was predicted to eventually level off with increasing light intensity because when light intensity was no longer a limiting factor of photosynthetic rate, other factors such as amount of water and CO2 became limiting.
This investigation involved determining the effect of light intensity on the rate of photosynthesis as measured by rate of oxygen production of spinach leaves. This measurement was taken by the determining the rate at which spinach segments floated in liquid as oxygen produced during photosynthesis diffused into liquid-filled intercellular spaces.
However, intercellular spaces of spinach leaves are largely gas-filled by nature. Thus, the spinach segments were first made to sink by subjecting a mixture of the leaves and 0.
The rate of leaf segments found floating parallel to the surface of the liquid not tilted was recorded as a measure of rate of photosynthesis. Incandescent light bulbs provided the light source for leaf segments to undergo photosynthesis. The leaves were submerged in solutions of NaHCO3 which were placed under the lamps.
In addition, clear vessels of water were positioned between the lamps and leaf mixtures as heat sinks to prevent the leaves from denaturing from the heat of the lamps.
The volume of water and size of the vessels used for these heat sinks were the same for each treatment to control temperature. The concentration of NaHCO3 was also held constant at 0.
Wavelength of light was uniform at white. All treatments were tested at room temperature. Size of leaf segments and number of leaf segments were also consistent for each treatment. To determine the effect of light intensity on rate of photosynthesis, the leaf mixtures were subjected to intensities of, and lux.
A treatment was performed in the dark 0 lux for the control. Leaf segments observed floating at 0 lux would indicate oxygen production from spontaneous reactions other than photosynthesis.
This would have been subtracted from the rates of photosynthesis of the other treatments to make the correction. The rate of oxygen production as measured by percent of floating leaf segments after five minutes was graphed against light intensity. In this experiment, number of leaf floating segments floating were counted at five minute intervals for 20 minutes.
Data from the first five minutes was graphed, because data collected after 5 minutes did not seem to would not adequately represent trends between light intensity and rate of photosynthesis.My Hypothesis was that if you placed the spinach disks in the highest concentration of bicarbonate then that is where photosynthesis would happen the fastest.
We cut out equal disk from a plant leaf and placed half in water and the other half a bicarbonate solution. Aim: The aim of this experiment is to investigate the effect of sodium carbonate and light intensity on the rate of oxygen produced by plants by observing the floating of the Spinach leaf.
Aim: The aim of this experiment is to investigate the effect of sodium carbonate and light intensity on the rate of oxygen produced by plants . Firstly, the leaf extract was prepared by weighting out 3 g of fresh spinach. After that the spinach was cut into small pieces and placed in a mortar, while 15 ml of ice-cold acetone and a sprinkle of clean sand were added.
Flat- or smooth-leaf spinach has broad, smooth leaves that are easier to clean than Savoy. This type is often grown for canned and frozen spinach, as well as soups, baby foods, and processed foods. Giant Noble is an example variety. Semi-savoy is a hybrid variety with slightly crinkled leaves.
It has the same texture as Savoy, but it is not as Family: Amaranthaceae. How scientists turned a spinach leaf into beating heart tissue The research may offer a solution to our donated organ shortage.