Estimating osmolarity by change in volume of potato tuber tissue

Use of this fact can be made in estimating the water potential of a plant tissue. Samples of tissue are immersed in a range of external solutions of different strengths.

Estimating osmolarity by change in volume of potato tuber tissue

Discussion If diffusion will not occur between the potato and sucrose solution at the same osmolarity then there will be a low percentage change in weight and volume between initial and final volume of the potato cylinder.

The results of the experiment support my hypothesis and prediction because there was a low percentage in weight change at 0.

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The potato is isotonic with a 0. If you look at graph 3 you can see that from 0.

Estimating osmolarity by change in volume of potato tuber tissue

The potato is hypertonic when compared to the sucrose solution at 0. This experiment was rather straight forward and I think the only major source of error would be human error with measurements. I am sure further familiarity with the calipers would minimize this error but they were rather new to us and measurements of length and diameter could of been more precise.

Another source of error that is out of our control is the accuracy of the sucrose solutions used in the experiment. Their potency could of been off or the bottle may have been labeled wrong.

How do you calculate osmolarity of a solution? | Socratic

Further research should be done on other vegetables or plants to find their ideal water content and isotonicity. Cell Physiology Source Book: Essentials of Membrane Biophysics.The mean volume (percent of total) of the different compartments of potato tuber tissue is shown in Figure 3.

The histograms of volume distributions show the . To reiterate, osmolarity is the total concentration of all the solute particles in a solution and tonicity is the ability of a solution to change the shape or tone of cells by altering their internal water volume (Marieb & /5(1).

Graph 3: Change in Initial and Final Volume of Potato Cylinders at Different Sucrose Osmolarities [pic 3] 5. Discussion. If diffusion will not occur between the potato and sucrose solution at the same osmolarity then there will be a low percentage change in weight and volume between initial and final volume of /5(1).

The volume of this tissue was measured as the volume of the precipitated fragments in an aqueous medium in a calibrated flask. Preparation of potato tuber tissue Ten tubers taken at random were peeled and diced by hand into small pieces, 3 “ 3 cm, and then mixed well.

In this way, by looking at the change in mass of the potato, one can be able to determine whether the solutions were hypertonic, hypotonic or isotonic to the potato samples and therefore find the osmolarity of the potatoes.

Potato Osmolarity Kimball Williams Marisol Garcia Abel Cuadras Thanh Tran Hypothesis If the potato is placed in a sucrose solution or , then it will be hypotonic.

If the potato is placed in a sucrose solution ranging from to , then it will be hypertonic.

Osmosis potato lab report - Great College Advice.